Fatima Arif  0357-Mphil-Bot-23 <br/><br/>
Biotechnological applications of ergot alkaloids produced by pleurotus ostreatus (jacq. Ex fr.) P. Kumm  English 
2023-25 
 - GC University Lahore 
 -  Mphil Thesis  CD 
<br/><br/> ABSTRACT<br/>The furrgal strain Plettrotns ostrealr1.r \.vas usecl in the present study which has the<br/>ability to prodttce ergot alkaloids. l-he ergot alkaloids production was achieved fiom<br/>the extracellular extract and intracellular extract of p. ostrealas using surface culture<br/>fermentation techniqr-re. various fermentation conclitions \.vere optimized such as<br/>effect nitrogen and carbon sources. other fermentation medium ingredients i.e. effect<br/>of KFizPo+, cacl:, ZnSo+. 1\4gSo+.7rIz o and FeSo+.7Hzo, and nrany process<br/>parameters i'e' effect of plJ level. incubation time period and inoculum sizes by using<br/>one factor at a time method (OFAT). Maximum yield of ergot alkaloids was obtained<br/>from the extracellular extracts at optimum level of sucrose (15%i),tryptone (20%i),<br/>KHzPo+ (2.5o/oi), cactu (2%). Znso.+ (r%). MgSor.THzo (0.0r%), IreSo+.7Hzo<br/>(0'05%), at pH 5'5 and 25oC after 5days of incubarion. The highest yield of ergot<br/>alkaloids was also achieved in the extracellular extract at incubation period (9days)<br/>and inoculum size (10m1). Afteroptirnization, one liter batch with optimize medium<br/>ingredients and fermentation conditions was run and yield of ergot alkaloicls r,vas<br/>l'352+0'03 mg/ml' Partial purification of ergot alkaloicls r,vas also perfbrrned using<br/>chloroform extraction method and rotatorv evaporator. The purified extract contain<br/>maximum quantity of ergot alkaloids (2.113+0.015 mg/ml). I'LC was perfbnned for<br/>the further analytical analysis of ergot alkaloids and it was found that {/r,alue (0.ga)<br/>fbr the extracellular extract r'vas attained in the rnobile phase F lr.hich indicating the<br/>presence of ergocrr''ptine alkaloids. Anti-firneal and ant-bacterial actir itr of ergot<br/>alkaloids r'vas also perfbrmed but no si-enillcant resLrhs riere lound. Ergot alkaloids<br/>was applied in rabbits as lor'r' concentration dose of lme and hieh concentration close<br/>of 5mg and then compared with controi. The bodr rieight of all rabbits \\ere not<br/>changed significantly. The symptoms of tail necrosis \\ere appeared on T2 rabbit. The<br/>HCT and platelets values of both rabbits (T1 and T2) u.ere decreased treated with<br/>ergot alkaloids while liver function of T2 was atTected. 
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