ABSTRACT
The furrgal strain Plettrotns ostrealr1.r \.vas usecl in the present study which has the
ability to prodttce ergot alkaloids. l-he ergot alkaloids production was achieved fiom
the extracellular extract and intracellular extract of p. ostrealas using surface culture
fermentation techniqr-re. various fermentation conclitions \.vere optimized such as
effect nitrogen and carbon sources. other fermentation medium ingredients i.e. effect
of KFizPo+, cacl:, ZnSo+. 1\4gSo+.7rIz o and FeSo+.7Hzo, and nrany process
parameters i'e' effect of plJ level. incubation time period and inoculum sizes by using
one factor at a time method (OFAT). Maximum yield of ergot alkaloids was obtained
from the extracellular extracts at optimum level of sucrose (15%i),tryptone (20%i),
KHzPo+ (2.5o/oi), cactu (2%). Znso.+ (r%). MgSor.THzo (0.0r%), IreSo+.7Hzo
(0'05%), at pH 5'5 and 25oC after 5days of incubarion. The highest yield of ergot
alkaloids was also achieved in the extracellular extract at incubation period (9days)
and inoculum size (10m1). Afteroptirnization, one liter batch with optimize medium
ingredients and fermentation conditions was run and yield of ergot alkaloicls r,vas
l'352+0'03 mg/ml' Partial purification of ergot alkaloicls r,vas also perfbrrned using
chloroform extraction method and rotatorv evaporator. The purified extract contain
maximum quantity of ergot alkaloids (2.113+0.015 mg/ml). I'LC was perfbnned for
the further analytical analysis of ergot alkaloids and it was found that {/r,alue (0.ga)
fbr the extracellular extract r'vas attained in the rnobile phase F lr.hich indicating the
presence of ergocrr''ptine alkaloids. Anti-firneal and ant-bacterial actir itr of ergot
alkaloids r'vas also perfbrmed but no si-enillcant resLrhs riere lound. Ergot alkaloids
was applied in rabbits as lor'r' concentration dose of lme and hieh concentration close
of 5mg and then compared with controi. The bodr rieight of all rabbits \\ere not
changed significantly. The symptoms of tail necrosis \\ere appeared on T2 rabbit. The
HCT and platelets values of both rabbits (T1 and T2) u.ere decreased treated with
ergot alkaloids while liver function of T2 was atTected.

Mphil Botany

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